CHES, 2-(Cyclohexylamino)ethane-1-sulfonic acid, is a buffering agent. CHES buffers have a useful range of pH 8.6–10.

It typically appears as a white crystalline powder.

Effect of impurities

Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes.

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