GLI may refer to:

• Getty Leadership Institute at Claremont Graduate University

• Glen Innes Airport, in New South Wales, Australia

• Global Labour Institute, a British workers rights organization

• Great Lakes Initiative, a fictional superhero team

• Great Lakes Invitational, a college ice hockey tournament

• Greater Louisville Inc., an American business development organization

• Greyhound Lines, a North-American bus carrier

• Volkswagen GLI, an automobile

• GLI1, GLI2, GLI3, glioma-associated family of transcription factors