The enzyme quercitrinase (, formerly 3.2.1.66) catalyzes the following chemical reaction:

1. quercitrin + H2 L-rhamnose + quercetin

Thus, the two substrates of this enzyme are quercitrin and H2O, whereas its two products are L-rhamnose and quercetin.

This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is quercitrin 3-L-rhamnohydrolase.

The enzyme can be found in Aspergillus flavus. It is an enzyme in the rutin catabolic pathway.

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