First Flight may refer to:

• Maiden flight, the first flight of a new aircraft type

  First Flight Airport, in North Carolina, United States

• First Flight High School, in Kill Devil Hills, North Carolina

• First Flight (sculpture), a sculpture in Milwaukee, Wisconsin

• First Flight (racehorse), 1940s champion racehorse

• First Flight (medical research horse), source of the first botulinum antitoxin

• First Flight Handicap, a horse race

• First Flight Tournament, a golf tournament in Japan

• First Flight (Star Trek: Enterprise), an episode of the television series

• First Flight (film), an animated short film

• Green Lantern: First Flight

• First Flight (novel), a novel by Chris Claremont

• First Flight: Maiden Voyages in Space and Time, a collection of short-stories

• Magik One: First Flight, a music compilation

It may also refer to:

• The first recorded powered, controlled flight of a heavier-than-air craft

• First solo flight

• First aerial circumnavigation