Nucleic acid methods are the techniques used to study nucleic acids: DNA and RNA.

Purification

• DNA extraction

• Phenol–chloroform extraction

• Minicolumn purification

• RNA extraction

• Boom method

• Synchronous coefficient of drag alteration (SCODA) DNA purification

Quantification

• Abundance in weight: spectroscopic nucleic acid quantitation

• Absolute abundance in number: real-time polymerase chain reaction (quantitative PCR)

• High-throughput relative abundance: DNA microarray

• High-throughput absolute abundance: serial analysis of gene expression (SAGE)

• Size: gel electrophoresis

Synthesis

• De novo: oligonucleotide synthesis

• Amplification: polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), transcription-mediated amplification (TMA)

Kinetics

• Multi-parametric surface plasmon resonance

• Dual-polarization interferometry

• Quartz crystal microbalance with dissipation monitoring (QCM-D)

Gene function

• RNA interference

Other

• Bisulfite sequencing

• DNA sequencing

• Expression cloning

• Fluorescence in situ hybridization

• Lab-on-a-chip

• Comparison of nucleic acid simulation software

• Northern blot

• Nuclear run-on assay

• Radioactivity in the life sciences

• Southern blot

• Differential centrifugation (sucrose gradient)

• Toeprinting assay

• Several bioinformatics methods, as seen in list of RNA structure prediction software

See also

• CSH Protocols

• Current Protocols

References

External links

• Protocols for Recombinant DNA Isolation, Cloning, and Sequencing