du Toit is an Afrikaans surname of French origin, originally from François du Toit, a Huguenot who moved to South Africa in 1686. It translates as "of the roof". People with the surname include:

• Alexander du Toit (1878–1948), South African geologist

• Anri du Toit aka Yolandi Visser, South African musician

• Braam du Toit (born 1981), South African composer

• Christiaan du Toit (1901–1982), South African military commander

• Daniel du Toit (1871–1959), South African astronomer

• Dirk du Toit (1943–2009), South African politician

• Elize du Toit (born 1981), South African born, British actress

• Flooi du Toit (1869–1909), South African cricketer

• Francois Jacobus du Toit (1897–1961), South African journalist and economist

• Gaffie du Toit (born 1976), South African rugby union

• Jacob Daniël du Toit (Totius, 1877–1953), South African poet and translator

• Jané du Toit (born 1975), Namibian rugby union footballer

• Lydia Lindeque (1916–1997), South African actor

• Natalie du Toit (born 1984), South African swimmer

• Nick du Toit, South African arms dealer

• Paul du Toit (1965–2014), South African artist

• Pieter-Steph du Toit (born 1992), South African rugby union player

• Rachel Alida du Toit (1916–1997), South African actor

• Sarel Petrus du Toit (1864–1930), Second Boer War Boer general

• Simoné du Toit (born 1988), South African shot putter

• Stephanus Jacobus du Toit (1847–1911), South African Afrikaans language pioneer

• Wikus du Toit (born 1972), South African actor

• Donald du Toit (born 1946), is a South African specialist general surgeon, and clinician

See also

• Du Toit Mountains, Antarctica

• Du Toits Peak, Western Cape, South Africa

• Du Toit's torrent frog, Arthroleptides dutoiti, species in the family Petropedetidae

• 57P/du Toit–Neujmin–Delporte, comet

• Du Toitcratercrater, on Mars

• Dutoitia, a genus of Devonian rhyniophyte, named after the South African geologist Alex du Toit