EOF or Eof may refer to:

Science and technology

• Electro-osmotic flow, the motion of liquid induced by an applied potential

• Empirical orthogonal functions, in statistics and signal processing

• Ethyl orthoformate, an organic compound

Computing

• End-of-file, a condition where no more data can be read from a data source

• End of frame of an Ethernet transmission

• Enterprise Objects Framework, a NeXT object-relational mapping product

• EoF, a song editing program for the free Guitar Hero clone Frets on Fire

Other uses

• Eof (about 701), a swineherd who claimed to have seen a vision of the Virgin Mary in England

• End of Fashion, an Australian band

• (EOF), an audiovisual work eligible for broadcasting and investment quotas in France